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Parametric study on the separation of extracellular vesicles in a sheathless spiral microfluidic device

Authors :

Mohammad Mahdi Abdi¹ Seyedeh Sarah Salehi²

1- دانشگاه علم و صنعت ایران 2- دانشگاه علم و صنعت ایران

Keywords :

microfluidic device،spiral microchannels،extracellular vesicles،particle separation

Abstract :

Extracellular vesicles which carry biological molecules including proteins, RNA, and DNA are emerging as promising biomarkers for non-invasive diagnostics. There are several conventional techniques such as centrifugation, precipitation, and filtration for the manipulation and analysis of extracellular vesicles. However, conventional methods often suffer from a lack of purity and require dedicated handling of trained personnel. In this study, an inertial microfluidic device is proposed to circumvent several steps and isolate extracellular vesicles from a blood sample. A finite element code is developed to solve the governing equations of motion and track particle trajectories. A two-dimensional spiral geometry is designed to passively isolate extracellular vesicles from red blood cells, white blood cells, and platelets by incorporating hemodynamic forces including inertial lift and drag forces. The results demonstrate that the separation efficiency of the microfluidic device is nearly 83% for extracellular vesicles from whole blood sample, indicating its potential for robust isolation of extracellular vesicles.