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Proposed Amniotic Membrane/Alginate Dialdehyde Based Injectable Hydrogel as a Biofunctional Scaffold for Soft Tissue Engineering

Authors :

Yasaman Pahlavanzadeh¹ Yousef Mohammadi² Maryam Saadatmand³

1- دانشگاه صنعتی شریف، تهران، ایران 2- دانشگاه صنعتی شریف، تهران، ایران 3- دانشگاه صنعتی شریف، تهران، ایران

Keywords :

Decellularized human amniotic membrane،Alginate dialdehyde،Injectable hydrogel،Tissue engineering،In situ formation،Cardiac tissue engineering

Abstract :

This study proposes a novel biofunctional hydrogel composed of decellularized human amniotic membrane (dHAM) and alginate dialdehyde (ADA) as a potential injectable scaffold for regenerative medicine. Human amniotic membranes (HAM) were decellularized, and histological evaluation using hematoxylin and eosin (H&E) staining, Masson’s trichrome (MT) staining, and Alcian blue (AB) staining confirmed the complete removal of cellular material while preserving extracellular matrix components such as collagen and glycosaminoglycans. ADA with a theoretical oxidation degree of 5% was synthesized using sodium metaperiodate, and its oxidation degree and oxidation yield were quantified by ultraviolet–visible spectrophotometry and iodometric titration, resulting in mean values of 3.58 ± 0.1% and 71.6 %, respectively. The composite hydrogel of dHAM and ADA was prepared via Schiff-base crosslinking between aldehyde groups of ADA and amino groups of solubilized dHAM, combined with ionic gelation mediated by calcium carbonate, calcium sulfate dihydrate, and glucono-δ-lactone. Thanks to its short gelation time (13.5±2.12 min), this formulation appears promising for in situ hydrogel formation applications. This hybrid hydrogel, introduced for the first time, retained the key biochemical and structural features of native extracellular matrix and demonstrated suitable gelation behavior, highlighting its potential as a promising candidate for tissue engineering applications.